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Badiranji Buya Keli, a Traditional Uyghur Medicine, Induces Vasodilation in Rat Artery: Signaling Mediated by Nitric Oxide Production in Endothelial Cells
Author(s) -
Miernisha A.,
Bi Cathy W. C.,
Cheng Lily K. W.,
Xing J. G.,
Liu J.,
Maiwulanjiang M.,
Aisa H. A.,
Dong Tina T. X.,
Lin Huangquan,
Huang Y.,
Tsim Karl W. K.
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5494
Subject(s) - enos , nitric oxide , umbilical vein , traditional medicine , vasodilation , rosmarinic acid , pharmacology , chemistry , medicine , endothelial nitric oxide synthase , nitric oxide synthase , biochemistry , in vitro , antioxidant
Badiranji Buya Keli (BBK) is a traditional Uyghur medicine derived from Dracocephalum Moldavica Herba (DMH, the aerial part of Dracocephalum moldavica L.). BBK has been widely used in treating cardiovascular and cerebrovascular diseases. Here, the quality control of BBK was established by using HPLC analysis of rosmarinic acid and tilianin. After chemical standardization, the biological effects of BBK was tested. First, BBK inhibited platelet aggregation of rabbit plasma. Second, BBK induced vasodilation in rat aortic ring, and this effect was partially mediated by nitric oxide (NO) production in endothelial cells. Third, BBK induced NO production in cultured human umbilical vein endothelial cells (HUVECs). In HUVECs, the phosphorylation of endothelial NO synthase (eNOS) was markedly increased after application of BBK. Pre‐treatment with the eNOS blocker N ω ‐nitro‐ l ‐arginine methyl ester hydrochloride could abolish BBK‐induced NO production and eNOS phosphorylation. Taken together, these results suggest that BBK could exert beneficial effects in cardiovascular system, which may provide parts of molecular explanation to account for its traditional usage in Uyghur medicine. Copyright © 2015 John Wiley & Sons, Ltd.