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Ex Vivo and In Situ Evaluation of ‘Dispelling‐Wind’ Chinese Medicine Herb–Drugs on Intestinal Absorption of Chlorogenic Acid
Author(s) -
Zhai Lixiang,
Shi Jun,
Xu Weitong,
Heinrich Michael,
Wang Jianying,
Deng Wenji
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5492
Subject(s) - chlorogenic acid , ex vivo , absorption (acoustics) , chemistry , herb , in vivo , bioavailability , pharmacology , traditional medicine , biochemistry , medicine , biology , in vitro , chromatography , medicinal herbs , materials science , microbiology and biotechnology , composite material
This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used ‘dispelling‐wind’ TCM botanical drugs [roots of Angelica dahurica (Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) and Saposhnikovia divaricata (Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance. Ex vivo everted intestinal sac and in situ single pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P‐gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the P app and K a of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P‐gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions. Copyright © 2015 John Wiley & Sons, Ltd.