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Potent Insulin Secretagogue from Scoparia dulcis Linn of Nepalese Origin
Author(s) -
Sharma Khaga Raj,
Adhikari Achyut,
Hafizur Rahman M.,
Hameed Abdul,
Raza Sayed Ali,
Kalauni Surya Kant,
Miyazaki JunIchi,
Choudhary M. Iqbal
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5412
Subject(s) - friedelin , secretagogue , insulin , betulinic acid , islet , chemistry , in vivo , phytotherapy , pancreatic islets , cell culture , pharmacology , biochemistry , traditional medicine , biology , endocrinology , medicine , stereochemistry , genetics , alternative medicine , microbiology and biotechnology , triterpenoid , pathology
Ethno‐botanical inspired isolation from plant Scoparia dulcis Linn. (Sweet Broomweed) yielded six compounds, coixol (1), glutinol (2), glutinone (3), friedelin (4), betulinic acid (5), and tetratriacontan‐1‐ol (6). There structures were identified using mass and 1D‐ and 2D‐NMR spectroscopy techniques. Compounds 1–6 were evaluated for their insulin secretory activity on isolated mice islets and MIN‐6 pancreatic β ‐cell line, and compounds 1 and 2 were found to be potent and mildly active, respectively. Compound 1 was further evaluated for insulin secretory activity on MIN‐6 cells. Compound 1 was subjected to in vitro cytotoxicity assay against MIN‐6, 3T3 cell lines, and islet cells, and in vivo acute toxicity test in mice that was found to be non‐toxic. The insulin secretory activity of compounds 1 and 2 supported the ethno‐botanic uses of S. dulcis as an anti‐diabetic agent. Copyright © 2015 John Wiley & Sons, Ltd.