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Salicin from Willow Bark can Modulate Neurite Outgrowth in Human Neuroblastoma SH‐SY5Y Cells
Author(s) -
Wölfle Ute,
Haarhaus Birgit,
Kersten Astrid,
Fiebich Bernd,
Hug Martin J.,
Schempp Christoph M.
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5400
Subject(s) - salicin , neurite , creb , mapk/erk pathway , sh sy5y , receptor , neuroblastoma , pharmacology , biology , microbiology and biotechnology , cell culture , chemistry , phosphorylation , transcription factor , biochemistry , in vitro , genetics , gene
Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH‐SY5Y. The functionality was analyzed in the neuroblastoma cell line SH‐SY5Y after stimulation with salicin, a known TAS2R16 agonist. In this setting salicin induced in SH‐SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that salicin might modulate neurite outgrowth by bitter taste receptor activation. Copyright © 2015 John Wiley & Sons, Ltd.