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New Activities for Isolated Compounds from Convolvulus austro‐aegyptiacus as Anti‐ulcerogenic, Anti‐ Helicobacter pylori and Their Mimic Synthesis Using Bio‐guided Fractionation
Author(s) -
Awaad Amani S.,
AlRifai Asmaa A.,
ElMeligy Reham M.,
Alafeefy Ahmed M.,
Zain Mohamed E.
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5379
Subject(s) - fractionation , toxicity , ethanol , acute toxicity , traditional medicine , biology , pharmacology , helicobacter pylori , chemistry , medicine , biochemistry , chromatography , organic chemistry , genetics
Bio‐guided fractionation of the total alcoholic extract of Convolvulus austro‐aegyptiacus was screened for its anti‐ulcerogenic activity, using an absolute‐ethanol‐induced ulcer model at 500 and 1000 mg/kg doses. Two compounds were isolated from the butanol extract of C. austro‐aegyptiacus and identified by 1 H and 13 C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50 mg/kg) showed a remarkable anti‐ulcerogenic activity because they exhibited control‐ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD 50) was more than 4000 mg/kg. Moreover, the obtained results were confirmed by the sub‐chronic toxicity because the rats that have been administered 1000 mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions. Copyright © 2015 John Wiley & Sons, Ltd.