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The Lignan Pinoresinol Induces Nuclear Translocation of DAF‐16 in Caenorhabditis elegans but has No Effect on Life Span
Author(s) -
Koch Karoline,
Büchter Christian,
Havermann Susannah,
Wätjen Wim
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5330
Subject(s) - lignan , pinoresinol , caenorhabditis elegans , biology , oxidative stress , biochemistry , mutant , chemistry , microbiology and biotechnology , botany , gene
The lignan pinoresinol is a constituent of flaxseed, sesame seeds and olive oil. Because of different molecular effects reported for this compound, e.g. antioxidative activity, pinoresinol is suggested to cause positive effects on humans. Because experimental data are limited, we have analysed the effects of the lignan on the nematode Caenorhabditis elegans : in spite of a strong antioxidative capacity detected in an in vitro assay, no antioxidative effects were detectable in vivo . In analogy to this result, no modulation of the sensitivity against thermal stress was detectable. However, incubation with pinoresinol caused an enhanced nuclear accumulation of the transcription factor DAF‐16 (insulin/IGF‐like signalling pathway). Using a strain with an enhanced oxidative stress level ( mev‐1 mutant), we clearly see an increase in stress resistance caused by this lignan, but no change in reactive oxygen species. Furthermore, we investigated the effects of pinoresinol on the life span of the nematode, but no modulation was found, neither in wild‐type nor in mev‐1 mutant nematodes. These results suggest that pinoresinol may exert pharmacologically interesting effects via modulation of the insulin‐like signalling pathway in C. elegans as well as in other species like mammals due to the evolutionary conservation of this signalling pathway. Copyright © 2015 John Wiley & Sons, Ltd.