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Antiinflammatory and Wound Healing Effects of Caesalpinia sappan L.
Author(s) -
Tewtrakul Supinya,
Tungcharoen Pattreeya,
Sudsai Teeratad,
Karalai Chatchanok,
Ponglimat Chanita,
Yodsaoue Orapun
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5321
Subject(s) - fibroblast , pharmacology , medicine , lipopolysaccharide , wound healing , traditional medicine , dermal fibroblast , tumor necrosis factor alpha , in vitro , chemistry , biochemistry , immunology
Extracted compounds from Caesalpinia sappan L. were examined for the inhibitory activity against NO, PGE 2 , and TNF‐α productions and on associated transcription levels using RAW264.7 cells. They were also tested for their effects on wound healing using fibroblast L929 cells. Among the compounds tested, brazilin (8) was the most effective against lipopolysaccharide (LPS)‐induced NO production in RAW264.7 cells with an IC 50 value of 10.3 μM, followed by sappanchalcone (2, 31.0 μM). Brazilin (8) also inhibited PGE 2 and TNF‐α production with IC 50 values of 12.6 and 87.2 μM, respectively. The antiinflammatory mechanism of brazilin involved down regulation of the mRNA expressions of the iNOS, COX‐2, and TNF‐α genes in a dose‐dependent manner. An ethanol (EtOH) extract of C. sappan significantly increased fibroblast proliferation, fibroblast migration, and collagen production, whereas brazilin (8) only stimulated fibroblast migration. In addition, the EtOH extract showed no acute toxicity in mice, and it was therefore safe to make use of its potent antiinflammatory and wound healing activities. Brazilin was mainly responsible for its antiinflammatory effect through its ability to inhibit the production of NO, PGE 2 , and TNF‐α. This study supports the traditional use of C. sappan for treatment of inflammatory‐related diseases. Copyright © 2015 John Wiley & Sons, Ltd.