Premium
The Anti‐Osteoporosis and Antioxidant Activities of Chemical Constituents from Chrysanthemum indicum Flowers
Author(s) -
Luyen Bui Thi Thuy,
Tai Bui Huu,
Thao Nguyen Phuong,
Lee Young Mi,
Lee Sang Hyun,
Jang Hae Dong,
Kim Young Ho
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5281
Subject(s) - chemistry , flavonoid , antioxidant , tartrate resistant acid phosphatase , scavenging , biological activity , food science , nuclear chemistry , traditional medicine , stereochemistry , organic chemistry , acid phosphatase , biochemistry , enzyme , in vitro , medicine
Two new compounds, chrysinoneside A (1) and (−)‐ trans ‐chrysanthenol‐6‐ O ‐ β ‐D‐glucopyranoside (2), along with 17 known compounds (3–19) were isolated from Chrysanthemum indicum flowers. The total phenolic and flavonoid contents of various fractions were determined. The EtOAC fraction had the highest total phenolic content (525.84 ± 23.51 mg GAE/g DR) and the total flavonoid content (63.49 ± 3.32 mg QE/g DR). The EtOAc and water fractions showed the greatest peroxyl radical‐scavenging capacity and the ability to reduce Cu(I) ions, with ORAC and CUPRAC values ranging from 24.00 ± 0.44 to 28.06 ± 1.35 and 16.90 ± 0.51 to 49.77 ± 0.97 μM, respectively. Compounds 5–11, 18, and 19 displayed strong effects in both peroxyl radical‐scavenging and reducing capacity assays at a concentration of 10 μM. The anti‐osteoporosis activity of these compounds was also evaluated. Compounds 10, 13, and 19 exhibited the most potent tartrate‐resistant acid phosphatase activity in receptor activator of nuclear factor‐κB ligand‐induced osteoclastic RAW 264.7 cells with values of 105.95 ± 1.18, 110.32 ± 3.95, and 112.58 ± 6.42%, respectively. Copyright © 2015 John Wiley & Sons, Ltd.