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Anti‐Proliferative Effects of Polyphenols from Pomegranate Rind ( Punica granatum L.) on EJ Bladder Cancer Cells Via Regulation of p53/miR‐34a Axis
Author(s) -
Zhou Benhong,
Yi Huilan,
Tan Jun,
Wu Yue,
Liu Gang,
Qiu Zhenpeng
Publication year - 2015
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5267
Subject(s) - viability assay , apoptosis , microrna , punica , polyphenol , cd44 , gene knockdown , cell growth , western blot , gene silencing , cancer research , cytotoxicity , cell , cancer cell , biology , chemistry , cancer , traditional medicine , biochemistry , medicine , in vitro , antioxidant , genetics , gene
miRNAs and their validated miRNA targets appear as novel effectors in biological activities of plant polyphenols; however, limited information is available on miR‐34a mediated cytotoxicity of pomegranate rind polyphenols in cancer cell lines. For this purpose, cell viability assay, Realtime quantitative PCR for mRNA quantification, western blot for essential protein expression, p53 silencing by shRNA and miR‐34a knockdown were performed in the present study. EJ cell treatment with 100 µg (GAE)/mL PRE for 48 h evoked poor cell viability and caspase‐dependent pro‐apoptosis appearance. PRE also elevated p53 protein and triggered miR‐34a expression. The c‐Myc and CD44 were confirmed as direct targets of miR‐34a in EJ cell apoptosis induced by PRE. Our results provide sufficient evidence that polyphenols in PRE can be potential molecular clusters to suppress bladder cancer cell EJ proliferation via p53/miR‐34a axis. Copyright © 2015 John Wiley & Sons, Ltd.