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Catechin‐based Procyanidins from Peumus boldus Mol. Aqueous Extract Inhibit Helicobacter pylori Urease and Adherence to Adenocarcinoma Gastric Cells
Author(s) -
Pastene Edgar,
Parada Víctor,
Avello Marcia,
Ruiz Antonieta,
García Apolinaria
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5176
Subject(s) - chemistry , catechin , proanthocyanidin , urease , gallic acid , helicobacter pylori , fractionation , high performance liquid chromatography , chromatography , aqueous solution , polyphenol , biochemistry , antioxidant , enzyme , biology , organic chemistry , genetics
In this work, the anti‐ Helicobacter pylori effect of an aqueous extract from dried leaves of Peumus boldus Mol. (Monimiaceae) was evaluated. This extract displayed high inhibitory activity against H . pylori urease. Therefore, in order to clarify the type of substances responsible for such effect, a bioassay‐guided fractionation strategy was carried out. The active compounds in the fractions were characterized through different chromatographic methods (RP‐HPLC; HILIC‐HPLC). The fraction named F5 (mDP = 7.8) from aqueous extract was the most active against H . pylori urease with an IC 50 = 15.9 µg gallic acid equivalents (GAE)/mL. HPLC analysis evidenced that F5 was composed mainly by catechin‐derived proanthocyanidins (LC‐MS and phloroglucinolysis). The anti‐adherent effect of boldo was assessed by co‐culture of H . pylori and AGS cells. Both the aqueous extract and F5 showed an anti‐adherent effect in a concentration‐dependent manner. An 89.3% of inhibition was reached at 2.0 mg GAE/mL of boldo extract. In conjunction, our results suggest that boldo extract has a potent anti‐urease activity and anti‐adherent effect against H . pylori , properties directly linked with the presence of catechin‐derived proanthocyanidins. Copyright © 2014 John Wiley & Sons, Ltd.