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Antiinflammatory Effects and Chemical Constituents of Veronicastrum axillare
Author(s) -
Zheng ChengJian,
Deng XueHong,
Wu Yu,
Jiang YiPing,
Zhu JianYong,
Qin LuPing
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5168
Subject(s) - chemistry , in vivo , ethyl acetate , apigenin , in vitro , lipopolysaccharide , biological activity , pharmacology , biochemistry , flavonoid , antioxidant , biology , microbiology and biotechnology , endocrinology
Our study aims to ascertain the antiinflammatory activity of Veronicastrum axillare and characterize the bioactive constituents. Antiinflammatory activity of the total extract and different fractions from V. axillare was investigated by employing the xylene‐induced mouse ear edema model. As a result, the ethyl acetate (EtOAc) fraction showed the highest antiinflammatory activity in vivo . From the EtOAc fraction and the inactive dichloromethane fraction, a total of five new compounds, axillasides A–C and axillactones A and B, together with four known compounds, procumboside A, buergeriside C 1 , indole‐3‐carboxylic acid and apigenin, were isolated and identified. Their structures were elucidated on the basis of spectroscopic analysis and by comparison of their nuclear magnetic resonance data with those reported in the literature. Procumboside A, a major constituent in EtOAc fraction, showed significant antiinflammatory activity in vivo . Further studies revealed that procumboside A was a potent COX‐2 inhibitor, significantly reducing the COX‐2 protein level in lipopolysaccharide‐stimulated RAW 264.7 macrophages. Copyright © 2014 John Wiley & Sons, Ltd.