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Anti‐leishmanial Activities of Extracts and Isolated Compounds from Drechslera rostrata and Eurotium tonpholium
Author(s) -
Awaad Amani S.,
AlZaylaee Haifa M.,
Alqasoumi Saleh I.,
Zain Mohamed E.,
Aloyan Ebtesam M.,
Alafeefy Ahmed M.,
Awad Elham S. H.,
ElMeligy Reham M.
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5096
Subject(s) - chemistry , anthraquinone , stereochemistry , traditional medicine , organic chemistry , medicine
The fungal extract of Drechslera rostrata and Eurotium tonpholium showed a significant anti‐leishmanial activity against Leishmania major ; IC 50 was 28.8 and 28.2 μ g/mL, respectively. Seven compounds, five from D. rostrata (H1–H5) and two from E. tonpholium (H6 and H7), were isolated and identified using different spectroscopic analysis including 1 HNMR, 13 CNMR, Hetero‐nuclear multiple bond connectivity (HMBC), Hetero‐nuclear Multiple Quantum Correlation (HMQC), and EI‐MS. The isolated compounds are: di ‐2‐ethylhexyl phthalate (1), (22E)‐5α,8α‐epidioxyergosta‐6,22‐diene‐3β‐ol (2),1,3,8‐trihydroxy‐6‐methyl‐nthraquinone (3), aloe‐emodine 8‐ O ‐glucopyranoside(4), 2R, 3R,4R,5R hexane 1, 2, 3, 4, 5, 6 hexole (Mannitol) (5), 1,8‐dihydroxy‐3‐methoxy‐6‐methyl‐anthraquinone (6) and 1, 4, 5‐trihydroxy‐7‐methoxy‐2‐methyl‐anthraquinone (7). However, compounds (1) and (6) showed activity against L. major with IC 50 of 3.2 and 10.38 µg/mL, respectively. On the other hand, oral administration of the two extracts (100 mg/kg) and compounds 1 and 6 (50 mg/kg) showed very good activity when compared with the anti‐leishmanial drug Pentostam (125 mg/kg). Interestingly, the complete heeling activity of the extracts and compounds (1) and (6) was obtained after 13–17 days of treatment, while complete healing activity of Pentostam was obtained after 28 days. No alteration on liver and kidney functions was recorded on animals treated with the two extracts for 15 consecutive days. Copyright © 2013 John Wiley & Sons, Ltd.