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Modulation of Melanin Synthesis by Rengyolone Isolated from the Root of Eurya emarginata in Melan‐a Cells
Author(s) -
Kim Jin Hee,
Jeong Sang Chul,
Hwang Jae Sung,
Lee Eun Sook,
Lee Sang Myung
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5082
Subject(s) - tyrosinase , melanin , enzyme , biochemistry , cytotoxicity , biology , lycorine , chemistry , botany , in vitro , alkaloid
In the course of screening for the melanogenesis inhibitors, rengyolone was isolated from Eurya emarginata (Thumb) Makino. Its chemical structure was determined on the basis of spectroscopic analysis including mass spectroscopy and nuclear magnetic resonance analysis. Rengyolone inhibited potent melanogenesis in melan‐a cells with an IC 50 value of 65 μM without cytotoxicity. Also, rengyolone showed a melanin biosynthesis inhibition zone in a culture plate of Streptomyces bikiniensis , which is commonly used as an indicator organism. Moreover, rengyolone dramatically reduced protein expression of melanogenic enzyme, tyrosinase. Furthermore, rengyolone presented inhibition on the body pigmentation in zebrafish model system and decreased melanin contents and tyrosinase activity. These results suggest that rengyolone isolated from E. emarginata may be an effective skin‐whitening agent that regulates the expression of melanogenic enzymes. Copyright © 2013 John Wiley & Sons, Ltd.