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Targeted Metabolomic Study Indicating Glycyrrhizin's Protection against Acetaminophen‐induced Liver Damage through Reversing Fatty Acid Metabolism
Author(s) -
Yu Jian,
Jiang YangShen,
Jiang Yuan,
Peng YanFang,
Sun Zhuang,
Dai XiaoNan,
Cao QiuTing,
Sun YingMing,
Han JingChun,
Gao YaJie
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5072
Subject(s) - glycyrrhizin , acetaminophen , pharmacology , chemistry , metabolomics , metabolism , biochemistry , medicine , chromatography
The present study aimed to give a short report on a possible mechanism of glycyrrhizin to acetaminophen‐induced liver toxicity. Seven‐day intraperitoneal administration of glycyrrhizin (400 mg/kg/day) to 2‐ to 3‐month‐old male C57BL/6N mice (mean weight 27 g) significantly prevents acetaminophen‐induced liver damage, as indicated by the activity of alanine transaminase and aspartate aminotransferase. Metabolomics analysis and principal component analysis (PCA) using ultra‐fast liquid chromatography coupled to triple time‐of‐flight mass spectrometer were performed. PCA separated well the control, glycyrrhizin‐treated, acetaminophen‐treated, and glycyrrhizin + acetaminophen‐treated groups. Long‐chain acylcarnitines were listed as the top ions that contribute to this good separation, which include oleoylcarnitine, palmitoylcarnitine, palmitoleoylcarnitine, and myristoylcarnitine. The treatment of glycyrrhizin significantly reversed the increased levels of long‐chain acylcarnitines induced by acetaminophen administration. In conclusion, this metabolomic study indicates a significant glycyrrhizin protection effect against acetaminophen‐induced liver damage through reversing fatty acid metabolism. Copyright © 2013 John Wiley & Sons, Ltd.