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Effect of Curcumin Against Pentylenetetrazol‐Induced Seizure Threshold in Mice: Possible Involvement of Adenosine A 1 Receptors
Author(s) -
Akula Kiran Kumar,
Kulkarni S. K.
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5048
Subject(s) - curcumin , adenosine , pentylenetetrazol , adenosine receptor , pharmacology , agonist , adenosine receptor antagonist , anticonvulsant , chemistry , seizure threshold , caffeine , adenosine a1 receptor , adenosinergic , antagonist , cgs 21680 , receptor , medicine , endocrinology , epilepsy , biochemistry , psychiatry
Curcumin, obtained from Curcuma longa , has been in use for manifold human disorders. The present study explores the effect of curcumin against pentylenetetrazol (PTZ) seizure threshold in mice. The possible involvement of adenosine receptor(s) mechanism was also investigated. Minimal dose of PTZ (i.v., mg/kg) needed to induce different phases of convulsions were recorded as an index of seizure threshold. Curcumin (20–120 mg/kg, p.o.) produced an increase in seizure threshold for convulsions induced by PTZ i.v. infusion. The anticonvulsant effect of curcumin (80 mg/kg) was prevented by 8‐phenyltheophylline (0.5 mg/kg, i.p., non‐selective adenosine receptor antagonist) and 8‐cyclopentyl‐1,3‐dipropylxanthine (5 mg/kg, i.p., adenosine A 1 receptor antagonist) but not by 8‐(3‐cholorostryl)caffeine (4 mg/kg, i.p., adenosine A 2A receptor antagonist). Further, 5′‐N‐ethylcarboxamidoadenosine (0.005 mg/kg, i.p., non‐selective A 1 /A 2 receptor agonist), or N 6 ‐cyclohexyladenosine (0.2 mg/kg, i.p., adenosine A 1 receptor agonist), was able to potentiate the anticonvulsant action of curcumin. In contrast, 5′‐(N‐cyclopropyl) carboxamidoadenosine (0.1 mg/kg, i.p., adenosine A 2A receptor agonist) failed to potentiate the effect of curcumin. This study demonstrated the anticonvulsant effect of curcumin against PTZ i.v. seizure threshold via a direct or indirect activation of adenosine A 1 but not A 2A receptors in mice. Thus, curcumin may prove to be an effective adjunct in treatment of convulsions. Copyright © 2013 John Wiley & Sons, Ltd.