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Salvia Miltiorrhiza Induces VEGF Expression and Regulates Expression of VEGF Receptors in Osteoblastic Cells
Author(s) -
Wenden Alex,
Yang Yanqi,
Chai Lei,
Wong Ricky W. K.
Publication year - 2014
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5031
Subject(s) - salvia miltiorrhiza , vascular endothelial growth factor , receptor , messenger rna , vegf receptors , microbiology and biotechnology , medicine , gene expression , endocrinology , chemistry , biology , pathology , biochemistry , gene , alternative medicine , traditional chinese medicine
This study investigated in vitro whether Salvia Miltiorrhiza Bunge (SM) induces vascular endothelial growth factor (VEGF) expression and regulates expression of VEGF receptors 1 (VEGFR‐1) and 2 (VEGFR‐2) on osteoblasts. MC3T3‐E1 cells were cultured with SM and VEGF at points 24, 48 and 72 h. A blank control was included. The mRNA expression of VEGF, VEGFR‐1 and VEGFR‐2 was examined using real‐time polymerase chain reaction. VEGF protein expression was examined using enzyme linked immunosorbent assay. SM increased VEGF mRNA expression by 21% at 24 h (p < 0.05), 5% (p < 0.05) at 48 h and 74% (p < 0.001) at 72 h, while external VEGF intervention decreased the internal VEGF expression by 51% at 24 h (p < 0.001) and increased it by 126% at 72 h (p < 0.001). SM increased VEGF protein at 72 h (p < 0.05). VEGFR‐1 mRNA expression, in response to SM, decreased by 8% (p < 0.05) at 72 h and decreased following VEGF at 24 and 72 h by 20% (p < 0.001) and 15%, respectively, (p < 0.001). VEGFR‐2 mRNA expression increased following SM at 24 and 48 h by 25% (p < 0.05) and 73% (p < 0.05), respectively, and decreased at 72 h by 13% (p < 0.05). It was concluded that SM modulated expression of VEGF and its receptors in MC3T3‐E1 cells. Copyright © 2013 John Wiley & Sons, Ltd.