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Ascorbigen Induces Dermal Papilla Cell Proliferation in Vitro , but Fails to Modulate Chemotherapy‐Induced Alopecia in Vivo
Author(s) -
Wang ChihHsiu,
Huang HsuShan,
Dai NiannTzyy,
Sheu MingJen,
Chang DehMing
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4933
Subject(s) - in vivo , dermal papillae , h&e stain , in vitro , cell growth , chemotherapy , pharmacology , pathology , medicine , staining , biology , chemistry , cancer research , hair follicle , microbiology and biotechnology , biochemistry
Ascorbigen (ABG) is the predominant indole‐derived compound from Brassica vegetables. In this study, we attempted to evaluate the effects of ABG on hair growth. To this end, we examined the proliferation of isolated human dermal papilla (DP) cells and keratinocytes after incubation in various concentrations (0–1.25 mM) of ABG. Furthermore, hair shaft regrowth was monitored in a mouse model of chemotherapy‐induced alopecia (CIA), and hematoxylin and eosin staining was performed for histological analyses. We found that 1.25 mM ABG induced a 1.2‐fold increase in the growth of DP cells, but not keratinocytes. However, ABG did not exert significant protective effects against CIA in the mouse model. These findings suggest that ABG may not be able to counteract CIA and that further investigation of the therapeutic potential of ABG in disease models is required. Copyright © 2013 John Wiley & Sons, Ltd.