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In vitro Anti‐diabetic Activity and Chemical Characterization of an Apolar Fraction of Morus alba Leaf Water Extract
Author(s) -
Hunyadi Attila,
Veres Katalin,
Danko Balazs,
Kele Zoltan,
Weber Edit,
Hetenyi Anasztazia,
Zupko Istvan,
Hsieh TustyJiuan
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4803
Subject(s) - dichloromethane , cinnamic acid , chemistry , fraction (chemistry) , chromatography , alcohol , gas chromatography–mass spectrometry , coniferyl alcohol , magnoliaceae , traditional medicine , organic chemistry , botany , mass spectrometry , biology , lignin , medicine , solvent
The tea from the white mulberry ( Morus alba L.) leaf is a worldwide known traditional medicine of type II diabetes. Here, we report the investigation of the dichloromethane‐soluble fraction obtained in a 0.24% m/m yield from the hot water extract of mulberry leaves. A significant, dose‐dependent activity was found by means of the 24‐h glucose consumption of fully differentiated adipocytes both in the absence and presence of insulin. The fraction was characterized by HPLC‐DAD, GC‐MS and GC‐FID. The main constituent (40.3% by means of GC‐FID) was isolated and identified as loliolide by EIMS, HRESIMS and NMR spectroscopy. In the active fraction benzyl alcohol, ethyl benzoate, t ‐cinnamic acid, p ‐hydroxyacetophenone, t ‐coniferyl alcohol and synapil alcohol were also identified by GC‐MS and quantified by GC‐FID (0.7, 1.3, 1.5, 2.9, 7.5 and 2.6%, respectively). Copyright © 2012 John Wiley & Sons, Ltd.