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High throughput Screening to Identify Natural Human Monoamine Oxidase B Inhibitors
Author(s) -
Mazzio E,
Deiab S,
Park K,
Soliman KFA
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4795
Subject(s) - monoamine oxidase b , traditional medicine , chemistry , glycyrrhiza uralensis , potency , tripterygium wilfordii , glycyrrhiza , pharmacology , biochemistry , monoamine oxidase , biology , enzyme , medicine , alternative medicine , pathology , in vitro
Age‐related increase in monoamine oxidase B (MAO‐B) may contribute to CNS neurodegenerative diseases. Moreover, MAO‐B inhibitors are used in the treatment of idiopathic Parkinson disease as preliminary monotherapy or adjunct therapy with L‐dopa. To date, meager natural sources of MAO‐B inhibitors have been identified, and the relative strength, potency and rank of many plants relative to standard drugs such as Selegiline (L‐deprenyl,Eldepryl) are not known. In this work, we developed and utilized a high throughput enzyme microarray format to screen and evaluate 905 natural product extracts (0.025‐.7 mg/ml ) to inhibit human MAO‐B derived from BTI‐TN‐5B1‐4 cells infected with recombinant baculovirus. The protein sequence of purified enzyme was confirmed using 1D gel electrophoresis‐matrix assisted laser desorption ionization ‐time‐of‐flight‐tandem mass spectroscopy, and enzyme activity was confirmed by [1] substrate conversion (3‐mM benzylamine) to H 2 0 2 and [2] benzaldehyde. Of the 905 natural extracts tested, the lowest IC 50 s [<0.07 mg/ml] were obtained with extracts of Amur Corktree ( Phellodendron amurense) , Bakuchi Seed(Cyamopsis psoralioides), Licorice Root (Glycyrrhiza glabra/uralensis) , Babchi (Psoralea corylifolia seed) . The data also show, albeit to a lesser extent, inhibitory properties of herbs originating from the mint family (Lamiaceae) and Turmeric, Comfrey, Bringraj, Skullcap, Kava‐kava, Wild Indigo, Gentian and Green Tea. In conclusion, the data reflect relative potency information by rank of commonly used herbs and plants that contain human MAO‐B inhibitory properties in their natural form. Copyright © 2012 John Wiley & Sons, Ltd.