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The Effect of the Ginkgo biloba Extract in the Expression of Bax, Bcl‐2 and Bone Mineral Content of Wistar Rats with Glucocorticoid‐Induced Osteoporosis
Author(s) -
Lucinda Leda Marília Fonseca,
Aarestrup Beatriz Julião Vieira,
Peters Vera Maria,
Paula Reis João Evangelista,
Oliveira Roberto Sotto Maior Fortes,
Oliveira Guerra Martha
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4747
Subject(s) - osteoporosis , ginkgo biloba , glucocorticoid , bone mineral , medicine , endocrinology , osteoblast , dental alveolus , bone density , tibia , chemistry , dentistry , pharmacology , in vitro , surgery , biochemistry
Objective: Evaluate the effects of the extract of Ginkgo biloba (EGb) in the glucocorticoid‐induced‐osteoporosis through the Bax and Bcl‐2 expressions by osteoblast cells, the x‐ray and bone density of the tibia. Method: Rats were divided into five groups: osteoporosis; EGb1 (28 mg/kg); EGb2 (56 mg/kg); alendronate (0.2 mg/animal) and control. The treatments were conducted for 20 ( n = 30) and 30 days ( n = 30). The Bax and Bcl‐2 expressions were evaluated in osteoblasts of the mandibular alveolar bone. The tibias were radiographed to evaluate the X‐ray and bone density. The control group was compared with the osteoporosis' (Student's t ‐test/Mann‐Whitney). The other groups were analyzed by analysis of variance test followed by Dunnett/Dunnett T3 ( p < 0.05). Results: When compared the osteoporosis to the control group ( p <0.05): Bax and x‐ray density increased; Bcl‐2 and the bone density reduced. When compared with the osteoporosis group ( p < 0.05), alendronate (30 days), EGb1 and EGb2 (20/30 days) increased the Bcl‐2 expression; EGb2 and alendronate (20 days) EGb1 and EGb2 (30 days) reduced the Bax expression; and EGb1 and EGb2 (20/30 days) reduced the X‐ray density. Conclusions: The EGb improved the Bcl‐2 and reduced the Bax expression by osteoblasts in the mandibular alveolar bone and recovered the mineral content in the tibia of rats with glucocorticoid‐induced‐osteoporosis. Copyright © 2012 John Wiley & Sons, Ltd.