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Investigation into the Genotoxicity of Water Extracts from Hypoxis Species and a Commercially Available Hypoxis Preparation
Author(s) -
Verschaeve L.,
Mertens B.,
Ndhlala A. R.,
Anthonissen R.,
Gorissen B.,
Van Staden J.
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4732
Subject(s) - genotoxicity , comet assay , chemistry , neutral red , pharmacognosy , cytotoxicity , traditional medicine , biology , in vitro , toxicity , dna damage , biochemistry , biological activity , dna , medicine , organic chemistry
We performed an in vitro evaluation of the genotoxic potential of water extracts from four Hypoxis species ( Hypoxis hemerocallidea, H. colchicifolia, H. rigidula, H. acuminata ) and a commercial preparation thereof using the neutral red uptake (NRU) assay, the alkaline comet assay and the cytome assay in human hepatoma HepG2 cells. The relative cytotoxicity of these samples was established by determining their NI 50 values (50% inhibition of NRU), and these results were used for dose‐finding in genotoxicity tests. None of the tested extracts were identified as genotoxic in both the alkaline comet assay and cytome assay. Copyright © 2012 John Wiley & Sons, Ltd.