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Promotion of Hair Growth by Rosmarinus officinalis Leaf Extract
Author(s) -
Murata Kazuya,
Noguchi Kazuma,
Kondo Masato,
Onishi Mariko,
Watanabe Naoko,
Okamura Katsumasa,
Matsuda Hideaki
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4712
Subject(s) - rosmarinus , finasteride , officinalis , hair growth , dihydrotestosterone , pharmacology , 5 alpha reductase inhibitor , androgen , lncap , minoxidil , pharmacognosy , chemistry , phytotherapy , androgen receptor , biological activity , medicine , traditional medicine , biochemistry , in vitro , prostate , physiology , alternative medicine , pathology , cancer , cancer cell , hormone , prostate cancer
Topical administration of Rosmarinus officinalis leaf extract (RO‐ext, 2 mg/day/mouse) improved hair regrowth in C57BL/6NCrSlc mice that experienced hair regrowth interruption induced by testosterone treatment. In addition, RO‐ext promoted hair growth in C3H/He mice that had their dorsal areas shaved. To investigate the antiandrogenic activity mechanism of RO‐ext, we focused on inhibition of testosterone 5α‐reductase, which is well recognized as one of the most effective strategies for the treatment of androgenic alopecia. RO‐ext showed inhibitory activity of 82.4% and 94.6% at 200 and 500 µg/mL, respectively. As an active constituent of 5α‐reductase inhibition, 12‐methoxycarnosic acid was identified with activity‐guided fractionation. In addition, the extract of R. officinalis and 12‐methoxycarnosic acid inhibited androgen‐dependent proliferation of LNCaP cells as 64.5% and 66.7% at 5 µg/mL and 5 μM, respectively. These results suggest that they inhibit the binding of dihydrotestosterone to androgen receptors. Consequently, RO‐ext is a promising crude drug for hair growth. Copyright © 2012 John Wiley & Sons, Ltd.