Premium
Macrophage Immunomodulatory Activity of a Purified Polysaccharide Isolated from Ganoderma atrum
Author(s) -
Yu Qiang,
Nie ShaoPing,
Li WenJuan,
Zheng WenYa,
Yin PengFei,
Gong DeMing,
Xie MingYong
Publication year - 2013
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4698
Subject(s) - polysaccharide , macrophage , phagocytosis , flow cytometry , internalization , fluorescein isothiocyanate , isothiocyanate , nitric oxide , nitric oxide synthase , microbiology and biotechnology , cell culture , biology , tumor necrosis factor alpha , chemistry , biochemistry , cell , immunology , in vitro , physics , genetics , quantum mechanics , fluorescence , endocrinology
The objective of this study was to evaluate the immunomodulatory effects of the purified Ganoderma atrum polysaccharide (PSG‐1) on murine macrophage cell line RAW264.7. Phagocytotic assay by fluorescein isothiocyanate‐dextran internalization showed that PSG‐1 stimulated the phagocytosis of macrophages. G. atrum polysaccharide increased the production of NO, and the level of mRNA expression of inducible nitric oxide synthase in a dose–response manner. G. atrum polysaccharide also dose‐dependently induced the release of TNF‐α and interleukin‐1β. Generation of reactive oxygen species was promoted by PSG‐1, as determined by flow cytometry. Moreover, PSG‐1 induced nuclear factor‐κB activation by elevation of p65 nuclear translocation, suggesting that PSG‐1 probably stimulated macrophage activities by activating the nuclear factor‐κB pathway. Copyright © 2012 John Wiley & Sons, Ltd.