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Acute Effect of High‐dose Isoflavones from Pueraria lobata (Willd.) Ohwi on Lipid and Bone Metabolism in Ovariectomized Mice
Author(s) -
Cho Hee Joon,
Jun Heejin,
Lee Ji Hae,
Jia Yaoyao,
Hoang Minh Hien,
Shim JaeHoon,
Park KwanHwa,
Lee SungJoon
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4669
Subject(s) - pueraria , ovariectomized rat , endocrinology , medicine , isoflavones , triglyceride , chemistry , phytotherapy , puerarin , estrogen , cholesterol , alternative medicine , pathology
We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β‐estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (−15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL‐fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans. Copyright © 2012 John Wiley & Sons, Ltd.