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Inhibitory Activities of Compounds from the Twigs of Garcinia hombroniana Pierre on Human Low‐density Lipoprotein (LDL) Oxidation and Platelet Aggregation
Author(s) -
Saputri Fadlina Chany,
Jantan Ibrahim
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4667
Subject(s) - chemistry , stigmasterol , friedelin , arachidonic acid , stereochemistry , inhibitory postsynaptic potential , in vitro , biochemistry , enzyme , chromatography , triterpenoid , endocrinology , biology
The methanol extract of the twigs of Garcinia hombroniana , which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone, 1,7‐dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin‐5‐en‐3β‐ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper‐mediated LDL oxidation and arachidonic acid (AA)‐, adenosine diphosphate (ADP)‐, collagen‐induced platelet aggregation in vitro . Among the compounds tested, 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone and 1,7‐dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half‐maximal inhibitory concentration (IC 50 ) values of 6.6 and 1.7 µ m , respectively. 3,5,3′,5′‐Tetrahydroxy‐4‐methoxybenzophenone exhibited strong activity on AA‐, ADP‐ and collagen‐induced platelet aggregation with IC 50 values of 53.6, 125.7 and 178.6 µ m , respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP‐induced aggregation with IC 50 value of 5.7 µ m . Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP‐induced platelet aggregation. Copyright © 2012 John Wiley & Sons, Ltd.