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Phorbol Ester or Epidermal Growth‐factor‐induced MUC5AC Mucin Gene Expression and Production from Airway Epithelial Cells are Inhibited by Apigenin and Wogonin
Author(s) -
Kim JuOck,
Sikder Md. Asaduzzaman,
Lee Hyun Jae,
Rahman Mustafizur,
Kim JangHyun,
Chang Gyu Tae,
Lee Choong Jae
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4650
Subject(s) - mucin , apigenin , epidermal growth factor , phorbol , microbiology and biotechnology , gene expression , chemistry , wogonin , biochemistry , biology , protein kinase c , gene , phosphorylation , flavonoid , medicine , pathology , receptor , antioxidant , scutellaria baicalensis , alternative medicine , traditional chinese medicine
In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12‐myristate 13‐acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI‐H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or EGF for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction (RT‐PCR) and enzyme linked immunosorbent assay (ELISA). The results were as follows: (i) apigenin and wogonin were found to inhibit the production of MUC5AC mucin protein induced by PMA or EGF; (ii) both compounds also inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. These results suggest that apigenin and wogonin can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells. Copyright © 2012 John Wiley & Sons, Ltd.