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3‐ O ‐Acetyloleanolic Acid Induces Apoptosis in Human Colon Carcinoma Hct‐116 Cells
Author(s) -
Yoo Ki Hyun,
Park JongHwa,
Cui En Ji,
Kim Kyung Il,
Kim Joo Yun,
Kim Jiyoung,
Hong Seong Gil,
Baek Nam In,
Chung In Sik
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.4616
Subject(s) - apoptosis , carcinoma , colon carcinoma , cancer research , medicine , biology , colorectal cancer , biochemistry , cancer
The cytotoxic effect of 3‐ O ‐acetyloleanolic acid, an oleanolic acid derivative isolated from the seeds of Vigna sinensis K., was investigated in human colon carcinoma HCT‐116 cells. 3‐ O ‐acetyloleanolic acid dose‐dependently inhibited the viability of HCT‐116 cells. Apoptosis was characterized by detection of cell surface annexin V and sub‐G1 apoptotic cell populations. The number of immunostained cells with annexin V‐FITC was increased after treatment with 3‐ O ‐acetyloleanolic acid. The sub‐G1 cell population was also increased. Expression of TRAIL‐mediated apoptosis signaling‐related death receptor DR5 was increased in 3‐ O ‐acetyloleanolic acid‐treated HCT‐116 cells. Activation of caspase‐8 and caspase‐3, critical mediators of extrinsic apoptosis signaling, was also increased by 3‐ O ‐acetyloleanolic acid. The results indicate that 3‐ O ‐acetyloleanolic acid induces apoptosis in HCT‐116 cells mediated by an extrinsic apoptosis signaling cascade via up‐regulation of DR5. Copyright © 2012 John Wiley & Sons, Ltd.