Premium
New Biological Activities of Casimiroa edulis Leaf Extract and Isolated Compounds
Author(s) -
Awaad Amani S.,
AlJaber Nabilah A.,
Soliman Gamal A.,
AlOuthman Mounerah R.,
Zain Mohamed E.,
Moses John E.,
ElMeligy Reham M.
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3690
Subject(s) - umbelliferone , phytochemical , imperatorin , sesquiterpene , chemistry , essential oil , biological activity , coumarin , terpene , traditional medicine , terpenoid , gas chromatography–mass spectrometry , chromatography , stereochemistry , mass spectrometry , biochemistry , organic chemistry , in vitro , medicine , high performance liquid chromatography
The phytochemical investigation of Casimiroa edulis Llave et Lex (Rotaceae) afforded four coumarins: umbelliferone (1), esculetin (2), imperatorin (3) and xanthotoxol (4). The identification of these compounds was achieved by using a combination of m.p., UV, EI‐mass, 1 H NMR and 13 C NMR spectroscopy. Essential oil extracts were analysed by GC/MS leading to the identification of 60 components. Sesquiterpene hydrocarbons accounted for the major make up of the oil. Microbiological screenings of the oil and successive plant fractions were performed, showing promising activity against a number of microorganisms with Minimum inhibitory concentrations (MIC) comparable to the standard antibiotics such as chloramphenicol and kanamycin. The plant ethanol extract (400 mg/kg) and the isolated coumarins (60 mg/kg) showed anticoagulant activity. Analyses to determine the activity of the extracts on liver and kidney function were performed, revealing no negative or detrimental effects. Copyright © 2011 John Wiley & Sons, Ltd.