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Cell‐Line Specific Protection by Berry Polyphenols Against Hydrogen Peroxide Challenge and Lack of Effect on Metabolism of Amyloid Precursor Protein
Author(s) -
Papandreou Magdalini A.,
Tsachaki Maria,
Efthimiopoulos Spiros,
KlimisZacas Dorothy,
Margarity Marigoula,
Lamari Fotini N.
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3670
Subject(s) - oxidative stress , chinese hamster ovary cell , biochemistry , chemistry , antioxidant , neuroprotection , polyphenol , mtt assay , cell culture , metabolism , microbiology and biotechnology , biology , pharmacology , cell growth , genetics , receptor
Amyloid precursor protein (APP) altered metabolism, Aβ‐overproduction/aggregation and oxidative stress are implicated in the development of Alzheimer's disease pathology. Based on our previous data indicating that administration of a polyphenol‐rich (PrB) blueberry extract (from wild Vaccinium angustifolium ) is memory enhancing in healthy mice and in order to delineate the neuroprotective mechanisms, this study investigated the antioxidant effects of PrB in H 2 O 2 ‐induced oxidative damage, Aβ peptide fibrillogenesis and APP metabolism. PrB suppressed H 2 O 2 ‐initiated oxidation (DCF assay) and cell death (MTT assay) in SH‐SY5Y cells. Protective effects were observed on Chinese hamster ovary (CHO) cells overexpressing APP770 carrying the mutation Val717Phe only at high concentrations, while further damage on HEK293 cells was induced after co‐treatment with 250 µ m H 2 O 2 and PrB in comparison with H 2 O 2 alone. Using the thioflavine T assay, blueberry polyphenols inhibited Aβ‐aggregation (~70%, 15 µg/mL) in a time‐dependent manner, while in the CHO APP770 cells it had no effect on APP metabolism as assessed by western blot. The results suggest that blueberry polyphenols exhibit antioxidant and/or pro‐oxidant properties according to the cellular environment and have no effect on APP metabolism. Copyright © 2011 John Wiley & Sons, Ltd.