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Inhibitory Activities of Pancreatic Lipase and Phosphodiesterase from Korean Medicinal Plant Extracts
Author(s) -
Lee Yun Mi,
Kim Young Sook,
Lee Youngseop,
Kim Junghyun,
Sun Hang,
Kim Joo Hwan,
Kim Jin Sook
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3644
Subject(s) - lipase , viscum album , phosphodiesterase , hydrolysis , chemistry , butyrate , traditional medicine , medicinal plants , enzyme , food science , biochemistry , medicine , fermentation
To find new pancreatic lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) inhibitors from natural products, 61 medicinal plants from Korea were screened for their antilipase activity for prevention of obesity. Dried and powdered plants were extracted three times with EtOH and extracts were obtained by removal of the solvent in vacuo . Lipase activity was determined by measuring the hydrolysis of p ‐nitrophenyl butyrate to p ‐nitrophenol. Also, the inhibitory effect was measured on phosphodiesterase (PDE), another therapeutic target for obesity. Of the extracts tested, Sorbus commixta (stem, leaf) and Viscum album (whole plant) exhibited antilipase activity (with IC 50 values of 29.6 µg/mL and 33.3 µg/mL, respectively) and only anti‐PDE activity (IC 50 values of 20.08 µg/mL and 35.15 µg/mL, respectively). Copyright © 2011 John Wiley & Sons, Ltd.