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Antibacterial Compounds from the Mushroom Ganoderma colossum from Nigeria
Author(s) -
Ofodile L. N.,
Uma N.,
Grayer R. J.,
Ogundipe O. T.,
Simmonds M. S. J.
Publication year - 2012
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3598
Subject(s) - mushroom , ganoderma , antimicrobial , bacteria , bacillus subtilis , dichloromethane , traditional medicine , antibacterial activity , agar , potency , gram positive bacteria , pharmacognosy , microbiology and biotechnology , chemistry , thin layer chromatography , biology , food science , chromatography , biological activity , ganoderma lucidum , medicine , biochemistry , in vitro , genetics , solvent
Three colossolactones (colossolactone E, colossolactone B and 23‐hydroxycolossolactone E) were isolated and characterized from an n ‐hexane:dichloromethane (2:7) extract of Ganoderma colossum using chromatographic techniques. The antimicrobial activity of the three compounds was then tested against Gram‐positive and Gram‐negative bacteria. The activity was evaluated by the thin‐layer chromatography agar overlay method. The results showed that colossolactone E and 23‐hydroxycolossolactone E were active against Bacillus subtilis and Pseudomonas syringae . Colossolactone B was not active against the bacteria. Their structures were elucidated by spectroscopic methods. Potency of the compounds against bacteria tested supports the use of this mushroom in therapeutic medicine. Copyright © 2011 John Wiley & Sons, Ltd.