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Inhibitory Effects of Costunolide Isolated from Laurus nobilis on IgE ‐induced Degranulation of Mast Cell‐like RBL‐2H3 Cells and the Growth of Y16 pro‐B Cells
Author(s) -
Kim Tae Joon,
Nam KungWoo,
Kim Bora,
Lee SungJin,
Oh KiBong,
Kim Kyeong Ho,
Mar Woongchon,
Shin Jongheon
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3553
Subject(s) - degranulation , immunoglobulin e , mast cell , biology , chemistry , microbiology and biotechnology , antibody , immunology , biochemistry , receptor
This study investigated the inhibitory effects of costunolide isolated from the leaves of Laurus nobilis L. (Lauraceae) on basophil‐mediated allergic reactions and interleukin (IL)‐5‐mediated B cell growth. The effects of costunolide on β‐hexosaminidase (a key parameter of degranulation) release and IL‐4 expression in rat basophilic leukemia (RBL‐2H3) cells were determined by measuring β‐hexosaminidase activity and by semi‐quantitative RT‐PCR, respectively. The effects of costunolide on Y16 pro‐B cell viability and growth were determined by 3‐(4,5‐dimethylthiazol‐2‐yl)2,5‐diphenyltetrazolium bromide (MTT) assay. Costunolide was found significantly to inhibit β‐hexosaminidase activity ( p < 0.01) and IL‐4 transcription in RBL‐2H3 cells in a dose‐dependent manner. Its 50% inhibitory concentration (IC 50 ) was 34 µ m , while that of the positive control, ketotifen, was 24 µ m (IL‐4 mRNA transcription). Moreover, costunolide dose‐dependently suppressed pro‐B cell growth in IL‐5‐stimulated Y16 cells. These results provide evidence that costunolide stabilizes mast cells by inhibiting IgE‐mediated degranulation and inhibits IL‐5‐stimulated B cell growth. Copyright © 2011 John Wiley & Sons, Ltd.