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Antiproliferative Action of Xylopia aethiopica Fruit Extract on Human Cervical Cancer Cells
Author(s) -
Adaramoye Oluwatosin A.,
Sarkar Jayanta,
Singh Neetu,
Meena Sanjeev,
Changkija Bendangla,
Yadav Prem P.,
Kanojiya Sanjeev,
Sinha Sudhir
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3551
Subject(s) - apoptosis , cytotoxicity , cell culture , cell cycle , fragmentation (computing) , cell cycle checkpoint , cell growth , cancer cell , dna fragmentation , programmed cell death , growth inhibition , biology , population , cell , cancer research , pharmacology , cancer , microbiology and biotechnology , medicine , biochemistry , in vitro , genetics , ecology , environmental health
The anticancer potential of Xylopia aethiopica fruit extract (XAFE), and the mechanism of cell death it elicits, was investigated in various cell lines. Treatment with XAFE led to a dose‐dependent growth inhibition in most cell lines, with selective cytotoxicity towards cancer cells and particularly the human cervical cancer cell line C‐33A. In this study, apoptosis was confirmed by nuclear fragmentation and sub‐G 0 /G 1 phase accumulation. The cell cycle was arrested at the G 2 /M phase with a decreased G 0 /G 1 population. A semi‐quantitative gene expression study revealed dose‐dependent up‐regulation of p53 and p21 genes, and an increase in the Bax/Bcl‐2 ratio. These results indicate that XAFE could be a potential therapeutic agent against cancer since it inhibits cell proliferation, and induces apoptosis and cell cycle arrest in C‐33A cells. Copyright © 2011 John Wiley & Sons, Ltd.