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Icariin induces the Expression of Toll‐like Receptor 9 in Ana‐1 Murine Macrophages
Author(s) -
Li Li,
Peng Lina,
Miao Jianhua,
Qiu Yafeng,
Zhou Ying,
Gao Xiaoqi,
Xu Yongli,
Shi Zixue,
Shao Donghua,
Ma Zhiyong
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3514
Subject(s) - icariin , stimulation , tumor necrosis factor alpha , tlr9 , receptor , pharmacology , innate immune system , immune system , medicine , immunology , chemistry , gene expression , biochemistry , alternative medicine , dna methylation , pathology , gene
Icariin is the major pharmacologically active compound of Herba epimedii which has been used as a tonic, aphrodisiac and an antirheumatic in traditional Chinese medicine. This study analysed the effect of icariin on the expression of Toll‐like receptor 9 (TLR9) which plays an important role in regulation of the innate immune response. Stimulation of Ana‐1 murine macrophages with icariin induced a significant dose‐dependent expression of TLR9, and its mRNA expression which increased from 3 h post‐treatment was approximately five‐fold that of DMSO‐treated cells. Several molecules, such as myeloid differentiation factor 88, tumor necrosis factor‐α and interleukin 6, which are involved in the TLR9 downstream signaling pathway, were also significantly up‐regulated in response to icariin stimulation. Our findings demonstrated that icariin is able to induce the expression of TLR9. Copyright © 2011 John Wiley & Sons, Ltd.