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Proteomic Analysis of Protective Effects of Polysaccharides from Salvia miltiorrhiza Against Immunological Liver Injury in Mice
Author(s) -
Sun XueGang,
Fu XiuQiong,
Cai HongBing,
Liu Qiang,
Li ChunHua,
Liu YaWei,
Li YingJia,
Liu ZhiFeng,
Song YuHong,
Lv ZhiPing
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3487
Subject(s) - salvia miltiorrhiza , liver injury , lipopolysaccharide , lactate dehydrogenase , chemistry , nitric oxide synthase , lipid peroxidation , biochemistry , nitric oxide , pharmacology , microbiology and biotechnology , immunology , biology , medicine , enzyme , pathology , alternative medicine , organic chemistry , traditional chinese medicine
This study was designed to investigate mechanisms of the protective effects of Salvia miltiorrhiza polysaccharide (SMPS) against lipopolysaccharide (LPS)‐induced immunological liver injury (ILI) in Bacille Calmette‐Guérin (BCG)‐primed mice. Two‐dimensional difference gel electrophoresis (2D‐DIGE) and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) analysis showed that three proteins are down‐regulated and six proteins are up‐regulated by SMPS. SMPS reduces the degree of liver injury by up‐regulating the enzymes of the citric acid cycle, namely malate dehydrogenase (MDH) and 2‐oxoglutarate dehydrogenase complex. LPS significantly increases nuclear factor kappa B (NF‐κB) activation, inducible nitric oxide synthase (iNOS) expression and MDA level in BCG primed mice liver, whereas SMPS treatment protects against the immunological liver injury through inhibition of the NF‐κB activation by up‐regulation of PRDX6 and the subsequent attenuation of lipid peroxidation, iNOS expression and inflammation. Copyright © 2011 John Wiley & Sons, Ltd.