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Protective Effect of Components Isolated from Lindera erythrocarpa against Oxidative Stress‐induced Apoptosis of H9c2 Cardiomyocytes
Author(s) -
Kim Jeong Ah,
Jung YiSook,
Kim MiYoung,
Yang Seo Young,
Lee Sohyun,
Kim Young Ho
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3465
Subject(s) - quercitrin , propidium iodide , oxidative stress , lactate dehydrogenase , chemistry , apoptosis , programmed cell death , biochemistry , pharmacology , stereochemistry , biology , antioxidant , enzyme , flavonoid
Eight compounds were isolated from the methanol fraction of Lindera erythrocarpa and assessed for their ability to protect H9c2 cardiomyocytes against oxidative stress‐induced cell death. Three of the compounds significantly reduced the release of lactate dehydrogenase from H9c2 cardiomyocytes treated with buthionine‐[S,R]‐sulfoximine and reduced the uptake of propidium iodide by these cells. These effects were concentration‐dependent. The three inhibitory compounds were identified as (−)‐epicatechin, avicularin and quercitrin by spectroscopic techniques including one‐ and two‐dimensional NMR and mass spectroscopy. Copyright © 2011 John Wiley & Sons, Ltd.