Premium
Ergosta‐7,22‐diene‐2β,3α,9α‐triol from the Fruit Bodies of Ganoderma lucidum Induces Apoptosis in Human Myelocytic HL‐60 Cells
Author(s) -
Lee Mi Kyoung,
Hung Tran Manh,
Cuong To Dao,
Na MinKyun,
Kim Jin Cheol,
Kim EunJung,
Park HeeSung,
Choi Jae Sue,
Lee IkSoo,
Bae KiHwan,
Hattori Masao,
Min Byung Sun
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3447
Subject(s) - apoptosis , dna fragmentation , poly adp ribose polymerase , mushroom , in vivo , pharmacology , cytotoxic t cell , caspase 3 , chemistry , in vitro , biology , biochemistry , medicine , traditional medicine , polymerase , dna , programmed cell death , botany , microbiology and biotechnology
Ganoderma lucidum is known as a medicinal mushroom used in traditional medicine. In our study, the cytotoxic activities of 17 compounds (1–17) isolated from the fruiting bodies of G. lucidum were investigated. Among them, ergosta‐7,22‐diene‐2β,3α,9α‐triol (EGDT) induced apoptosis in HL‐60 human premyelocytic leukemia cells. EGDT activated the apoptotic process, including DNA fragmentation and caspase‐3 activity. In immunoblotting analysis, treatment with EGDT resulted in the cleavage of procaspase‐3 and poly(ADP‐ribose) polymerase (PARP) into active forms. In the in vivo study, the administration (i.p.) of EGDT to Lewis lung carcinoma (LLC)‐inoculated mice evidenced a significant inhibition of tumor growth. These results indicate that EGDT was one of the apoptotic constituents of G. lucidum , and might be an antitumor agent. Copyright © 2011 John Wiley & Sons, Ltd.