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In Vitro Antiinflammatory Activity of a New Sesquiterpene Lactone Isolated from Siegesbeckia glabrescens
Author(s) -
Li Hua,
Kim Jae Yeon,
Hyeon Jinseong,
Lee Hwa Jin,
Ryu JaeHa
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3420
Subject(s) - nitric oxide synthase , nitric oxide , sesquiterpene , in vitro , lactone , lipopolysaccharide , cell culture , chemistry , biochemistry , biology , microbiology and biotechnology , stereochemistry , immunology , genetics , organic chemistry
A new sesquiterpene lactone was isolated from Siegesbeckia glabrescens as an inhibitor of nitric oxide and prostaglandin E 2 synthesis in the LPS‐activated RAW264.7 macrophage cell line. It dose‐dependently decreased the protein and mRNA levels of inducible nitric oxide synthase and cyclooxygenase‐2. Reporter gene assay revealed that the compound attenuated the LPS‐induced DNA transcriptional activity of nuclear factor‐kappa B (NF‐κB). In addition, it inhibited the nuclear translocation of the p65 subunit of NF‐κB by blocking the inhibitory kappa B‐α degradation. This compound from Siegesbeckia glabrescens might be beneficial for the treatment of inflammation‐related diseases. Copyright © 2011 John Wiley & Sons, Ltd.