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Hepatoprotective Effect of Flavonoid Glycosides from Lespedeza cuneata against Oxidative Stress Induced by tert ‐Butyl Hyperoxide
Author(s) -
Kim Sang Min,
Kang Kyungsu,
Jho Eun Hye,
Jung YuJin,
Nho Chu Won,
Um ByungHun,
Pan CheolHo
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3387
Subject(s) - isovitexin , traditional medicine , chemistry , flavonoid , quercetin , vitexin , glycoside , ethyl acetate , oxidative stress , ethanol , antioxidant , biochemistry , stereochemistry , medicine
The aerial parts of Lespedeza cuneata G. Don, perennial legume native to Eastern Asia, have been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, little is known about the pharmaceutical effect of extracts from this plant. In the present study, the aerial parts of L. cuneata were used to prepare an ethanol extract, which was then tested for hepatoprotective effects against injury by tert ‐butyl hyperoxide ( t ‐BHP). At a dose of 20 μg/mL, the ethanol extract significantly protected HepG2 cells against the cytotoxicity of t ‐BHP. Further fractionation of the extract with ethyl acetate allowed the isolation of five flavonoid compounds that were structurally identified by 1 H and 13 C NMR spectroscopy as isovitexin, hirsutrin, trifolin, avicularin and quercetin. Hirsutrin, avicularin and quercetin (10 μ m ) showed clear hepatoprotective activity against injury by t ‐BHP in HepG2 cells, whereas isovitexin and trifolin showed no protective effects. The observed hepatoprotective effect of the investigated compounds showed a high correlation with radical scavenging activity, which followed the structure–activity relationships of the flavonoid aglycones. Copyright © 2011 John Wiley & Sons, Ltd.