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Paeoniflorin protects against NMDA‐induced neurotoxicity in PC12 cells via Ca 2+ antagonism
Author(s) -
Mao QingQiu,
Zhong XiaoMing,
Li ZhaoYi,
Huang Zhen
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3321
Subject(s) - antagonism , paeoniflorin , neurotoxicity , nmda receptor , pharmacology , pharmacognosy , chemistry , medicine , toxicity , biological activity , high performance liquid chromatography , in vitro , biochemistry , chromatography , receptor , organic chemistry
Preclinical and clinical investigation has shown that hippocampal neuronal atrophy and destruction can be observed in patients with depression, and this can be ameliorated with antidepressant medication. Neuroprotection has therefore been proposed as one of the mechanisms of action of antidepressants. Paeoniflorin, a monoterpene glycoside, has been reported to display antidepressant‐like effects in animal models of behavioral despair. The present study aimed to examine the protective effect of paeoniflorin treatment on N ‐methyl‐ d ‐aspartate (NMDA)‐induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. Paeoniflorin was shown to elevate cell viability, decrease lactate dehydrogenase (LDH) release in NMDA‐treated PC12 cells. Paeoniflorin also reversed the increased intracellular calcium (Ca 2+ ) concentration and the reduced Calbindin‐D28K mRNA level caused by NMDA in PC12 cells. These results suggest that paeoniflorin exerts a neuroprotective effect on NMDA‐induced neurotoxicity in PC12 cells, at least in part, via Ca 2+ antagonism. Copyright © 2010 John Wiley & Sons, Ltd.