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Effects of diallyl sulphide in chondrocyte and cartilage in experimental osteoarthritis in rabbit
Author(s) -
Chen WeiPing,
Tang JingLi,
Bao JiaPeng,
Hu PengFei,
Yu Chong,
Shi ZhongLi,
Wu LiDong
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3267
Subject(s) - cartilage , chondrocyte , osteoarthritis , matrix metalloproteinase , anterior cruciate ligament , in vivo , chemistry , messenger rna , matrix (chemical analysis) , medicine , anatomy , pathology , biology , biochemistry , microbiology and biotechnology , alternative medicine , chromatography , gene
Diallyl sulphide (DAS) is known for its antioxidant, anticancer and detoxifying properties. The aim of this study was to investigate the effect of DAS on rabbit articular chondrocytes and cartilage in experimental osteoarthritis (OA) induced by anterior cruciate ligament transection (ACLT). DAS inhibited matrix metalloproteinase‐1 (MMP‐1), MMP‐3 and MMP‐13 expression in interleukin‐1beta (IL‐1β)‐induced chondrocytes. In an in vivo study, DAS ameliorated cartilage degradation as assessed by morphological and histological examination. Messenger RNA expression of MMP‐1, MMP‐3, MMP‐13 and IL‐1β was inhibited by DAS in cartilage. In addition, DAS increased the collagen II level in cartilage. The results suggest that DAS may protect cartilage in the development of OA. Copyright © 2010 John Wiley & Sons, Ltd.