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JAK2/STAT5 signaling pathway mediates Bojungbangdocktang enhanced hematopoiesis
Author(s) -
Lim Jeonghan,
Jeong SooJin,
Koh Wonil,
Han Ihn,
Lee HyoJung,
Kwon TaeRin,
Jung Ji Hoon,
Kim Jung Hyo,
Lee HyoJeong,
Lee EunOk,
Kim SunHyung,
Lee MinHo,
Kim SungHoon
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3257
Subject(s) - haematopoiesis , erythropoietin , bone marrow , stem cell factor , hematopoietic growth factor , thrombopoietin , biology , stem cell , erythropoiesis , stromal cell , cytokine , aplastic anemia , stat5 , janus kinase 2 , cancer research , immunology , medicine , endocrinology , signal transduction , microbiology and biotechnology , anemia
Abstract Bojungbangdocktang (BJBDT) is a medicinal herbal cocktail that has been used for cancer prevention and treatment in traditional Korean medicine. In the current study, BJBDT was demonstrated to regulate hematopoiesis. BJBDT significantly increased the expression of hematopoietic cytokines interleukin (IL)‐3, stem cell factor (SCF), granulocyte‐macrophage‐colony stimulating factor (GM‐CSF), thrombopoietin (TPO) and erythropoietin (EPO) at the level of mRNA and secretion in hematopoietic stem cells (HSCs). Additionally, BJBDT enhanced the phosphorylation of Janus activated kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) and STAT binding to gamma interferon activated sites (GAS) in HSCs. Furthermore, BJBDT significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony‐forming units (CFU‐GEMM) and erythroid burst forming units (BFU‐E) in vitro . Moreover, BJBDT increased the level of EPO at mRNA in kidney and plasma, and the numbers of erythroid‐specific antigen Ter‐119 + erythroid cells in mice with aplastic anemia induced by 20% benzene. Consistently, histochemical staining revealed BJBDT increased the bone marrow and stromal cells as well as decreased macrophages and adipocytes in bone marrow tissues of mice with aplastic anemia. Taken together, the results suggest that BJBDT can enhance hematopoiesis via hematopoietic cytokine‐mediated JAK2/STAT5 pathway as a potent hematopoietic candidate. Copyright © 2010 John Wiley & Sons, Ltd.