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Anti‐complement activity of isolated compounds from the roots of Clerodendrum bungei Steud.
Author(s) -
Kim SooKi,
Cho SangBuem,
Moon HyungIn
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3254
Subject(s) - verbenaceae , acetone , classical complement pathway , complement system , chemistry , pharmacognosy , biological activity , stereochemistry , traditional medicine , biology , biochemistry , botany , in vitro , medicine , immunology , immune system
Abstract To determine the anti‐complement activity of natural diterpenes, chromatographic separation of the acetone‐soluble fraction from the roots of Clerodendrum bungei (Verbenaceae) led to the isolation of five diterpenoids. An acetone‐soluble extract of the roots of C. bungei exhibited significant anti‐complement activity on the classical pathway complement system, which was expressed as total hemolytic activity. Five compounds isolated from the roots of C. bungei , namely 12‐ O ‐ β ‐ d ‐glucopyranosyl‐3,11,16‐trihydroxyabieta‐8,11,13‐triene (1), 3,12‐ O ‐ β ‐ d ‐diglucopyranosyl‐11,16‐dihydroxyabieta‐8,11,13‐triene (2), ajugaside A (3), uncinatone (4) and 19‐hydroxyteuvincenone F (5). Compounds 1, 2, 3, 4 and 5 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC 50 ) values of 24 µ m , 138 µ m , 116 µ m , 87 µ m and 232 µ m . Among the compounds tested, 1 showed the most potent anti‐complement activity (IC 50 , 24 µ m ). Copyright © 2010 John Wiley & Sons, Ltd.