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Antimicrobial activity of terpenoids from Copaifera langsdorffii Desf. against cariogenic bacteria
Author(s) -
Souza Ariana B.,
Martins Carlos H. G.,
Souza Maria G. M.,
Furtado Niege A. J. C.,
Heleno Vladimir C. G.,
de Sousa João P. B.,
Rocha Erilda M. P.,
Bastos Jairo K.,
Cunha Wilson R.,
Veneziani Rodrigo C. S.,
Ambrósio Sérgio R.
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3244
Subject(s) - streptococcus mutans , streptococcus sobrinus , antimicrobial , lactobacillus casei , terpenoid , chemistry , streptococcus mitis , microbiology and biotechnology , traditional medicine , bacteria , biology , food science , biochemistry , streptococcus , medicine , fermentation , genetics
In the present work, the anticariogenic activities of nine labdane type‐diterpenes and four sesquiterpenes were investigated. Among these metabolites, (‐)‐copalic acid (CA) was the most active compound displaying MIC values very promising (ranging from 2.0 to 6.0 µg/mL) against the main microorganisms responsible for dental caries: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis and Lactobacillus casei . Time kill assays performed with CA against the primary causative agent ( S. mutans ) revealed that, in the first 12 h, this compound only inhibits the growth of the inoculum (bacteriostatic effect). However, its bactericidal effect is clearly noted thereafter (between 12 and 24 h). Also, CA did not show a synergistic effect when combined with the anticariogenic gold standard (chlorhexidine, CHD) in the checkerboard assays against S. mutans . In conclusion, the results points out CA as an important metabolite in the search for new effective anticariogenic agents. Copyright © 2010 John Wiley & Sons, Ltd.