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Chloroform extract of aged black garlic attenuates TNF‐α‐induced ROS generation, VCAM‐1 expression, NF‐κB activation and adhesiveness for monocytes in human umbilical vein endothelial cells
Author(s) -
Lee Eun Na,
Choi Young Whan,
Kim Hye Kyung,
Park Jin Kyeong,
Kim Hyo Jin,
Kim Myoung June,
Lee Hee Woo,
Kim KiHyung,
Bae Sun Sik,
Kim Bong Seon,
Yoon Sik
Publication year - 2011
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3230
Subject(s) - umbilical vein , tumor necrosis factor alpha , vcam 1 , allium sativum , cell adhesion molecule , reactive oxygen species , nf κb , monocyte , chemistry , u937 cell , pharmacology , e selectin , icam 1 , cell adhesion , microbiology and biotechnology , biochemistry , biology , immunology , signal transduction , in vitro , cell , botany
Aged black garlic is a type of fermented garlic ( Allium sativum ) which has been used in Oriental countries for a long time because of various biological properties of garlic derivatives. The current study explored the potential of the chloroform extract of aged black garlic (CEABG) in attenuating the activities of adhesion molecules in tumor necrosis factor‐α (TNF‐α)‐stimulated human umbilical vein endothelial cells (HUVECs). The study was performed on HUVECs that were pretreated with 30 μg/mL of CEABG before TNF‐α treatment. Treatment of HUVECs with CEABG significantly inhibited TNF‐α‐induced reactive oxygen species (ROS) formation. HUVECs treated with CEABG showed markedly suppressed TNF‐α‐induced mRNA expression of VCAM‐1, but little alteration in ICAM‐1 and E‐selectin mRNA expression. CEABG treatment also significantly decreased the TNF‐α‐induced cell surface and total protein expression of VCAM‐1 without affecting ICAM‐1 and E‐selectin expression. In addition, treatment of HUVECs with CEABG markedly reduced THP‐1 monocyte adhesion to TNF‐α‐stimulated HUVECs. Furthermore, CEABG significantly inhibited NF‐κB transcription factor activation in TNF‐α‐stimulated HUVECs. The data provide new evidence of the antiinflammatory properties of CEABG that may have a potential therapeutic use for the prevention and treatment of vascular diseases such as atherosclerosis through mechanisms involving the inhibition of VCAM‐1 expression and NF‐κB activation in vascular endothelial cells. Copyright © 2010 John Wiley & Sons, Ltd.