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Bioactivity‐guided isolation of antiproliferative compounds from Centaurea arenaria
Author(s) -
Csapi Bence,
Hajdú Zsuzsanna,
Zupkó István,
Berényi Ágnes,
Forgo Peter,
Szabó Pál,
Hohmann Judit
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3187
Subject(s) - apigenin , hela , sesquiterpene , chemistry , mtt assay , biochemistry , biology , traditional medicine , cell growth , stereochemistry , in vitro , flavonoid , medicine , antioxidant
The antiproliferative effects of n ‐hexane, chloroform and aqueous methanol extracts prepared from the whole plant of Centaurea arenaria M.B. ex Willd. were investigated against cervix adenocarcinoma (HeLa), breast adenocarcinoma (MCF7) and skin epidermoid carcinoma (A431) cells, using the MTT assay. The chloroform extract displayed high tumour cell proliferation inhibitory activity (higher than 85% at 10 μg/mL concentration), and was therefore subjected to a bioassay‐guided multistep separation procedure. Flavonoids (eupatilin, eupatorin, 3′‐methyleupatorin, apigenin and isokaempferid), lignans (arctigenin, arctiin and matairesinol), the sesquiterpene cnicin, serotonin conjugates (moschamine and cis ‐moschamine), β‐amyrin and β‐sitosterin‐β‐D‐glycopyranoside, identified by means of UV, MS and NMR spectroscopy, were obtained for the first time from this species. The isolated compounds were also evaluated for their tumour cell growth inhibitory activities on HeLa, MCF7 and A431 cells, and different types of secondary metabolites were found to be responsible for the antitumour effects of the extracts; in addition to moderately active compounds (isokaempferid and moschamine), especially apigenin, eupatorin, arctigenin, arctiin, matairesinol and cnicin exert marked antitumour effects against these cell lines. Copyright © 2010 John Wiley & Sons, Ltd.