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Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes
Author(s) -
de Melo Cristiane Moutinho Lagos,
de Castro Maria Carolina Accioly Brelaz,
de Oliveira Andresa Pereira,
Gomes Fabiana Oliveira Santos,
Pereira Valéria Rêgo Alves,
Correia Maria Tereza Santos,
Coelho Luana Cassandra Breitenbach Barroso,
Paiva Patrícia Maria Guedes
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3156
Subject(s) - concanavalin a , splenocyte , lectin , cytotoxic t cell , in vitro , nitric oxide , biology , mixed lymphocyte reaction , cytotoxicity , microbiology and biotechnology , lymphocyte , immunology , chemistry , biochemistry , immune system , t cell , endocrinology
Cramoll 1,4 is a lectin extracted from Cratylia mollis Mart. seeds that has shown antitumor and lymphocyte mitogenic activities in other studies. The aim of this work was to investigate, in vitro , the immunomodulatory activity of Cramoll 1,4 on experimental cultures of mice lymphocytes through cytotoxic assays, nitric oxide (NO) concentrations and IL‐10 and IFN‐γ production. Cramoll 1,4 did not show cytotoxic activity at 1–25 μg/mL concentrations, similar results were observed with concanavalin A (Con A) and phytohemagglutinin (PHA) lectins. The minimum production of IL‐10 was observed in splenocytes cultivated with Con A, PHA and Cramoll 1,4 lectins. However, splenocytes treated with Cramoll 1,4 showed higher IFN‐γ production in comparison with PHA and Con A ( p < 0.05 for both). Production of NO was effectively suppressed in murine cells stimulated with the lectins and was only detected after 72 h for PHA in relation to non‐stimulated lymphocytes ( p < 0.05). Cramoll 1,4 was not toxic to murine lymphocytes, induced Th1 response through IFN‐γ production and showed antiinflammatory activity through NO suppression. Therefore, Cramoll 1,4 can be considered a lectin with immunomodulatory activity. Copyright © 2010 John Wiley & Sons, Ltd.