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A new antitumor arabinopyranoside from Laurencia majuscula induces G2/M cell cycle arrest
Author(s) -
Du Bin,
Zhong Xueyun,
Liao Xiaojian,
Xu Wenjie,
Zhou Xulong,
Xu Shihai
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3153
Subject(s) - cyclin dependent kinase 1 , cyclin b1 , cell cycle , cyclin , blot , flow cytometry , chemistry , microbiology and biotechnology , biology , cell , biochemistry , gene
A new arabinopyranoside was isolated from the alga Laurencia majuscula (Harvey) Lucas, collected from the Xisha Islands in the South China Sea. Its structure was elucidated as hexadecyl‐1‐O‐α‐ l ‐arabinopyranoside by spectroscopic analysis. It was found that arabinopyranoside had significant antitumor activity in LOVO and Bel‐7402 cell lines. Flow cytometric analysis showed that arabinopyranoside arrested the cell cycle in the G2/M phase. Western blotting demonstrated that the protein expression of CDK1 and cyclin A related to the G2/M phase decreased markedly with arabinopyranoside treatment, with slight changes in cyclin B1 expression. Taken together, the findings identify a potential new antitumor therapeutic arabinopyranoside isolated from red alga Laurencia majuscula . Copyright © 2010 John Wiley & Sons, Ltd.