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Three diterpenes from Leonurus japonicus houtt protect primary cultured rat cortical cells from glutamate‐induced toxicity
Author(s) -
Moon HyungIn
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3144
Subject(s) - toxicity , pharmacology , glutamate receptor , chemistry , cortical neurons , diterpene , traditional medicine , biology , medicine , biochemistry , neuroscience , receptor , organic chemistry
Abstract To examine the cytoprotective effects of Leonurus japonicus Houtt, its protection against the glutamate‐induced toxicity in primary cortical cultured neurons was tested. In order to clarify the cytoprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. By such fractionation, three known diterpenes – prehispanolone (1), leojaponin (2) and iso‐preleoheterin (3) – were isolated from the methanol extracts from the air‐dried L. japonicus . Among these three compounds, leojaponin (2) exhibited significant cytoprotective activities against glutamate‐induced toxicity, exhibiting cell viability of about 50%, at concentrations ranging from 0.1 μ m to 10 μ m . Therefore, the cytoprotective effect of L. japonicus might be due to the inhibition of glutamate‐induced toxicity by the diterpenes it contains. Copyright © 2010 John Wiley & Sons, Ltd.