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Inhibitory effects of Hoelen extract on melanogenesis in B16/F1 melanoma cells
Author(s) -
Chang Mun Seog,
Choi Moon Jung,
Park Soo Yeon,
Park Seong Kyu
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3123
Subject(s) - tyrosinase , melanin , microphthalmia associated transcription factor , depigmentation , melanoma , hyperpigmentation , blot , skin hyperpigmentation , microbiology and biotechnology , skin whitening , chemistry , biology , biochemistry , pharmacology , gene , enzyme , cancer research , genetics , active ingredient
Melanin synthesis is regulated by melanogenic proteins, such as tyrosinase, tyrosinase‐related protein 1 (TRP‐1) and TRP‐2. The effects of Hoelen extract on melanogenesis were investigated in B16Fl murine melanoma cells. Specifically, tyrosinase activity, cell viability and melanin content were assayed, and western blotting and RT‐PCR for tyrosinase, TRP‐1 and TRP‐2 conducted. The results show that Hoelen significantly inhibited melanin synthesis through inhibition of TRP‐2 expression, while it did not affect tyrosinase activity or its expression. Taken together, RT‐PCR results showed that the depigmentation effect of Hoelen may be due to inhibition of TRP‐2 gene transcription. These results suggest that Hoelen may be a useful inhibitor for the attenuation of melanogenesis and hyperpigmentation in skin cells. Copyright © 2010 John Wiley & Sons, Ltd.